Diffusion

Diffusion refers to the process by which molecules intermingle as a result of their kinetic energy of random motion. Consider two containers of gas A and B separated by a partition. The molecules of both gases are in constant motion and make numerous collisions with the partition. If the partition is removed as in the lower illustration, the gases will mix because of the random velocities of their molecules. In time a uniform mixture of A and B molecules will be produced in the container.

The tendency toward diffusion is very strong even at room temperature because of the high molecular velocities associated with the thermal energy of the particles.

Osmosis

If two solutions of different concentration are separated by a semi-permeable membrane which is permeable to the smaller solvent molecules but not to the larger solute molecules, then the solvent will tend to diffuse across the membrane from the less concentrated to the more concentrated solution. This process is called osmosis.

Osmosis is of great importance in biological processes where the solvent is water. The transport of water and other molecules across biological membranes is essential to many processes in living organisms. The energy which drives the process is usually discussed in terms of osmotic pressure.

The decision about which side of the membrane to call "high" osmotic pressure is a troublesome one. The choice made here is the opposite of that made in many biology texts, which attribute "high" osmotic pressure to the solution and zero osmotic pressure to pure water. The rationale for the choice is that the energy which drives the fluid transfer is the thermal energy of the water molecules, and that energy density is higher in the pure solvent since there are more water molecules. The thermal energy of the solute molecules does not contribute to transport, presuming that the membrane is impermeable to them. The choice is also influenced by the observed direction of fluid movement, since under this choice the fluid transport is from high "pressure" to low, congruent with normal fluid flow through pipes from high pressure to low. The final rationale has to do with the measurement of osmotic pressure by determining how much hydrostatic pressure on the solution is required to prevent the transport of water from a pure source across a semi-permeable membrane into the soluton. A positive pressure must be exerted on the solution to prevent osmotic transport, again congruent with the concept that the osmotic pressure of the pure solvent is relatively "high".

Nevertheless, the dialog continues on this issue since the discussion of osmosis is most relevant to the biological and life sciences and perhaps the logic stated above should yield to the conventions of the field in which the phenomena are most relevant.

Measuring Osmotic Pressure

One approach to the measurement of osmotic pressure is to measure the amount of hydrostatic pressure necessary to prevent fluid transfer by osmosis.